Monitoring antibacterial permeabilization in real time using time-resolved flow cytometry

Real-time monitoring for bioaerosols--flow cytometry.

Bioaerosol detection in real time is an urgent civilian and military requirement. In this article, bioaerosol mass spectrometry, an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence, real-time qPCR, and FCM/FL were discussed. Although, challenging work remains to determine the interfering substances (e.g. par...

Monitoring of Dynamic Microbiological Processes Using Real-Time Flow Cytometry

We describe a straightforward approach to continuously monitor a variety of highly dynamic microbiological processes in millisecond resolution with flow cytometry, using standard bench-top instrumentation. Four main experimental examples are provided, namely: (1) green fluorescent protein expression by antibiotic-stressed Escherichia coli, (2) fluorescent labeling of heat-induced membrane damag...

Real-time fluorescence and deformability cytometry — flow cytometry goes mechanics

Cell mechanical characterization has recently approached the throughput of conventional flow cytometers. However, this very sensitive, label-free approach still lacks the specificity of molecular markers. Here we combine real-time 1D-imaging fluorescence and deformability cytometry (RT-FDC) to merge the two worlds in one instrument — opening many new research avenues. We demonstrate its utility...

Broad Applications of Multi-Colour Time-Resolved Flow Cytometry

Real Time Monitoring using sEMG

A Wearable Electromyography device includes the advantage of Electromyography (EMG) sensors and provides a wired or wireless human machine interface (HMI) for interacting with computing systems and attached devices associated with electrical signals generated by specific movement of the patient’s muscles. Following initial automated self-calibration and positional localization processes, measur...